|
KMID : 0363320190400030425
|
|
Journal of Korean Oriental Internal Medicine
2019 Volume.40 No. 3 p.425 ~ p.442
|
|
|
Pharmacological Properties of CDBT in Hypoxia-induced Neuronal Cell Injury and Their Underlying Mechanisms
|
|
Park Sang-Kyu
Jung Eun-Sun
Cha Ji-Yoon
Cho Hyun-Kyoung
Yoo Ho-Ryong
Kim Yoon-Sik
Seol In-Chan
|
|
|
Abstract
|
|
|
Objectives: This study aimed to reveal the pharmacological properties of the newly prescribed herbal mixture, Chenmadansam- gamibokhap-tang (CDBT), against hypoxia-induced neuronal cell injury (especially mouse hippocampal neuronal cell line, HT-22 cells) and their corresponding mechanisms.
Methods: A cell-based in vitro experiment, in which a hypoxia condition induced neuronal cell death, was performed. Various concentrations of the CDBT were pre-treated to the HT-22 cells for 4 h before 18 h in the hypoxia chamber. The glial cell BV-2 cells were stimulated with IFNγ and LSP to produce inflammatory cytokines and reactive oxygen species. When the neuronal HT-22 cells were treated with this culture solution, the drug efficacy against neuronal cell death was examined.
Results: CDBT showed cytotoxicity in the normal condition of HT-22 cells at a dose of 125 μg/mL and showed a protective
effect against hypoxia-induced neuronal cell death at a dose of 31.3 μg/mL. CDBT prevented hypoxia-induced neuronal cell death in a dose-dependent manner in the HT-22 cells by regulating HIF1α and cell death signaling. CDBT prevented neuronal cell death signals and DNA fragmentation due to the hypoxia condition. CDBT significantly reduced cellular oxidation, cell death signals, and caspase-3 activities due to microglial cell activations. Moreover, CDBT significantly ameliorated LPS-induced BV-2 cell activation and evoked cellular oxidation through the recovery of redox homeostasis.
Conclusions: CDBT cam be considered as a vital therapeutic agent against neuronal cell deaths. Further studies are required to reveal the other functions of CDBT in vivo or in the clinical field.
|
|
|
KEYWORD
|
|
|
herbal medicine, hypoxia, cell death, neuronal oxidation, HT-22 cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|